High titre BacMAM viruses improve transduction efficiency of mammalian cells

•Transient production of proteins in mammalian cells is fundamental to studies on gene function in health and disease.
•Many viral and plasmid vectors are available to enable the transfer of genes into mammalian cells, including baculovirus vectors.
•Baculoviruses are insect-specific viruses that can transduce but not replicate in many mammalian cells. These BacMAM vectors utilise mammalian promoters to drive expression of target genes.
•One disadvantage of the current BacMAM system is that relatively high multiplicities of infection (50-200+ virus particles per cell) are often required for effective transduction
•This requires either concentration of the BacMAM virus (time-consuming/labour intensive) or the use of chemical enhancers.


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